Question 3 :
Which of the followingis the first hormone prepared by genetic engineering?<br>

Question 5 :
The organism, which is used for gene transfer in higher organism is

Question 7 :
Ampicillin-sensitive bacteria are exposed to a plasmid containing the gene for resistance to the antibiotic ampicillin. They are then spread on two agar growth plates. Plate A contains growth medium only. Plate B contains growth medium and ampicillin.<br/>Which plate might be expected to have cultures of ampicillin-resistant bacteria growing on it?

Question 10 :
Transfer of genetic material into a bacterial cell through a viral vector is known as<br/>

Question 13 :
Which of these is used as vector in gene therapy for SCID?

Question 14 :
In india, research in genetic modification of organisms and safety issues are controlled by

Question 15 :
Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague.Where would you find the smallest segments of DNA?<br>

Question 19 :
Why the production of a human protein in bacteria by genetic&#160;engineering became possible?<br/>

Question 20 :
The cutting of DNA at specific locations became possible with the discovery of<br/>

Question 22 :
Which of the following enzyme's other name is knife of DNA?

Question 25 :
The usual source of restriction endonucleases used in gene cloning is

Question 26 :
In the isolation of DNA, removal of protein and RNA is carried out by enzymes _____ and _________&#160;respectively.<br/>

Question 27 :
Which one of the following palindromic base sequences in DNA, can be easily cut at about the middle by some particular restriction enzyme?

Question 28 :
Which of the following option is correct for recombinant DNA technology?<br>

Question 30 :
Which is the first biochemical to be produced commercially by microbialcloning and genetic engineering ?<br>

Question 31 :
A gene whose expression helps to identify transformed cell is known as

Question 34 :
A scientist is trying to engineer a plasmid for purposes of genetically modifying crops.<br/>What is the most appropriate first step in the process?

Question 38 :
How many fragments will be generated in total upon digestion of a closed circular DNA molecule and liner DNA molecule with a same restriction enzyme having six recognition sites on both DNA molecules?

Question 40 :
Find the odd one among the following methods of rDNA transfer into host cell.

Question 41 :
Given below is a sample of portion of DNA strand given the base sequence on the opposite strand. What is so special show in it?<br/>5' - GAATTC ... 3'<br/>3' - CTTAAG ... 5'

Question 42 :
Which one of the following is a recognition site for restriction enzyme Bam HI?<br>

Question 44 :
How many fragments will be generated on the digestion of a closed circular DNA molecule with a&#160;restriction enzyme having six recognition sites on the DNA?

Question 45 :
How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having $3$ sites?

Question 46 :
Manipulation of DNA in genetic engineeringbecame possible due to the discovery of : -

Question 48 :
A bacterium modifies its DNA by adding methyl groups to the DNA, It does so to

Question 49 :
In the nomenclature of enzyme restriction endonuclease, the roman numeral indicates<br/>

Question 53 :
Amongst the following four statements (A-D) some are wrong.<br/><br/>A)&#160;Unarmed pathogen vectors are also used in the transfer of recombinant DNA into the host.<br/>B)&#160;Restriction enzymes are used in isolation of DNA from other macro-molecules.<br/>C)&#160;Downstream processing is one of the steps of separating recombinant DNA from other macro-molecules.<br/>D)The first transgenic sheep, Rosie produced enriched milk with human $\alpha$-lactalbumin.<br/>Which are the two statements with mistakes?

Question 57 :
Which of the following contains the key tools for recombinant DNA technology?<br>(i) Restriction endonucleases, ligases, vectors(ii) Ligases, host organism, polymerase enzymes(iii) Vectors, Taq polymerase, primers(iv) Restriction exonucleases, ligases, primers, bioreactors<br>

Question 58 :
Which of the following steps should be performed by a person in order to visualise the bands of DNA fragments obtained from gel electrophoresis?<br>

Question 59 :
Which of the following DNA sequences qualifies to be designated as a palindrome?<br>

Question 61 :
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.

Question 62 :
Plants are more readily manipulated by genetic engineering than are animals because

Question 63 :
A piece of nucleic acid used to find a gene by forming a hybrid with it, is called as<br/>

Question 64 :
Bacteria uses restriction endonuclease to protectitself from viral attack. The bacterial DNA does not get degraded by its own enzyme because

Question 65 :
Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzymes. Reason: In $pBR 322$ alien $DNA$ is ligated generally in the area of $Bam-HI$ site of tetracycline resistance gene.

Question 66 :
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilities the action of the enzyme DNA polymerase.

Question 67 :
In a cloning experiment using E.coli, a DNA fragment was inserted in an EcoRI restriction site of a plasmid vector that contained the $kan^{R}$ and $spec^{R}$ genes for resistance to the antibiotics kanamycin and spectinomycin, respectively. It was observed that all the positive clones (containing the DNA fragment of interest) grew on medium with kanamycin but not on media containing spectinomycin. Colonies, which grew on media with both antibiotics, did not contain the fragment of interest. In the absence of any other confounding factors, the statement(s) which could independently explain these observations is/are

Question 69 :
Which of the following DNA sequences qualifies to be designated as a palindrome?

Question 74 :
The nuclease enzyme which begins its attack fromfree end of a polynucleotide is

Question 75 :
For transformation, micro-particles coated with DNA to be bombarded with biolistic gene gun are made up of<br/>