Question 1 :
Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason: Both bacteria and yeast multiply very fast to form huge population which expresses the desired gene.
Question 4 :
Which of the following contains the key tools for recombinant DNA technology?<br>(i) Restriction endonucleases, ligases, vectors(ii) Ligases, host organism, polymerase enzymes(iii) Vectors, Taq polymerase, primers(iv) Restriction exonucleases, ligases, primers, bioreactors<br>
Question 5 :
Restriction endonuclease cuts DNA at specific sites and cellular DNA is not damaged as
Question 6 :
Which of the following DNA sequences qualifies to be designated as a palindrome?<br>
Question 8 :
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilities the action of the enzyme DNA polymerase.
Question 10 :
Nobel Prize of 1978 for restriction endonuclease technology was given to
Question 14 :
Restriction endonuclease, an enzyme used in genetic engineering is employed for<br/>
Question 15 :
In biotechnology application, a selectable marker is incorporated in a plasmid
Question 17 :
Genomic DNA is digested with Alu $I$, a restriction enzyme which is a four base - pair cutter. What is the frequency with which it will cut the DNA assuming a random distribution of bases in the genome?<br/>
Question 18 :
The restriction endonuelease EcoR-1 recognises and cleaves DNA sequence as shown below <br>5' - G A A T T C - 3' <br>3' - CT T A A G - 5' <br>What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence ?
Question 19 :
The sticky ends of a fragmented DNA molecule are made up of
Question 20 :
Assertion: Restriction endonuclease recognize short palindromic sequence and cut at specific sites.
Reason: When a restriction endonuclease acts on palindrome, it cleaves both the strands of DNA molecule.
Question 21 :
Which of the following DNA sequences qualifies to be designated as a palindrome?
Question 23 :
A piece of nucleic acid used to find a gene by forming a hybrid with it, is called as<br/>
Question 25 :
The new strain of bacteria produced by biotechnology in bioremediation is
Question 26 :
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
Question 27 :
Plasmids useful in genetic engineering are cut at specific sites by<br/>
Question 28 :
For transformation, micro-particles coated with DNA to be bombarded with biolistic gene gun are made up of<br/>
Question 29 :
Metabolic engineering is the branch of biotechnology for improvement of cellular activities by manipulation of enzymatic, transport and regulatory functions of cells using recombinant DNA technology. Still this science has limitations due to<br>
Question 30 :
Which of the following is the advantage of meiotic recombination in diploids?
Question 34 :
Which one of the following represents a palindromic sequence in DNA?
Question 36 :
Assertion: Recognition site should be preferably single and responsive to commonly used restriction enzymes.
Reason: In $pBR 322$ alien $DNA$ is ligated generally in the area of $Bam-HI$ site of tetracycline resistance gene.
Question 37 :
Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason: Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.
Question 38 :
Assertion: Restriction enzymes of different organisms that recognize the identical sequences are called isoschizomers.
Reason: They are present only in eukaryotes.
Question 40 :
An enzyme that cleaves DNA recognizes an 8 base-pair unique DNA sequence. The probable number of times this enzyme will cleave a 70 kilobase pair random DNA sequence is
Question 42 :
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
Question 43 :
Amongst the following four statements (A-D) some are wrong.<br/><br/>A) Unarmed pathogen vectors are also used in the transfer of recombinant DNA into the host.<br/>B) Restriction enzymes are used in isolation of DNA from other macro-molecules.<br/>C) Downstream processing is one of the steps of separating recombinant DNA from other macro-molecules.<br/>D)The first transgenic sheep, Rosie produced enriched milk with human $\alpha$-lactalbumin.<br/>Which are the two statements with mistakes?
Question 47 :
How many fragments will be generated if you digest a linear DNA molecule with a restriction enzymehaving four recognition sites on the DNA?
Question 50 :
The nuclease enzyme which begins its attack fromfree end of a polynucleotide is