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UNIT 4 Revision exam, 1) ——-—-—-- of the following is used to transfer the desired DNA into Host, i) E.coli ii) Virus iti) Plamid, A) Onlyi B)Ontyii C)Onlyiii DB) Bothiand ii, 2) -—------ is not a character of cloning vector, A)Self replication, B)Unique restriction site for restriction enzymes, C)Easily isolated, D)it should not have marker gene, 3) DNA cloning is achieved through -——-—-- of the following, , i)Cell based cloning, ii)Cell free cloning, , A)Onlyi 8)Onlyii C)Bothiandii 0) None of above, , 4)--------= enzymes used to cut the DNA fragment, A)Phosphatase B)Exonucleases C)llgase D)Endonucleases, , §)---—- enzyme af the fallowing is used to construct cDNA library, A) Reverse Transcriptase 8) DNAPOL! C)Slnuclease D) All of above, , 6)--------- enzyme is involved with formation of complementary DNA, A)DNA Pol! 8) 51 Nuclease C) Reverse Transcriptase D) RE, , 7)DNA fingerprinting is based on - -- of the following, A) Thumb Printing 8) Change in repetitive sequences C) Cloning of fragments D) All of, above, , , , 8)In mammal cloning the nucleus is taken fram ——---A) Ovum B) Somatic cells of mammary epithelium C) Uterus D) Ovary, , 9)The introduction exogenous DNA into genome of an organism is known as -----—--—A)Cloning 8) Transgenesis C)DNA fingerprinting D) Gene Transfer, , 10) Delta endotoxin is isolated fromm --------=, A) E coli B) Bsubtilis C)B,Thuringiensis 0) 8. clausi