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Revision exam On Unit 1, , 1) Sugar puckering In B DNA, A)C3endo 8B)C2 endo, C)C3Exo D)C2 Exo, , 2)DNa is stable than RNa, A)More B)Less C)Equal D) None, , 3)In eukaryotes, which of the following DNA polymerase is required for, mitochondrial DNA replication?, , a) Pol alpha, , b) Pol beta, , c) Pol gamma, , d) Pol delta, , 4)In eukaryotes, which of the following DNA polymerase is highly processive and, required for the elongation phase of DNA replication?, , a) Pol alpha, , b) Pol beta, , c) Pol gamma, , d) Pol delta, , 5). Which of the following are the properties of enzyme telomerase?, , a) Telomerase is a reverse transcriptase enzyme, , b) Telomerase consist of an RNA sequence that serves as a template, , c) After completion of each cycle, telomerase translocates to 3'end of DNA to, synthesize repetitive sequence., , d) All of the above, , 6)As the two strands of the double helix are separated, the positive supercoiling, interferes with the further unwinding of DNA. Which of the following enzyme, makes a break in a strand of DNA to release the supercoiling and facilitate the, replication to occur?, , a) DnaA protein, , b) Single-strand binding protein, , c) DNA polymerase, , d) Topoisomerase, , 7)The DNA polymerase is a template-directed enzyme that synthesizes new, complementary strand from a parent strand but it requires the existing short, nucleotide sequence for its elongation. Which of the following enzyme is required, for the synthesis of this primer?, , a) Primase/RNA polymerase, , b) RNA synthase

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c) DNA synthase, d) Helicase, , 8) DNA polymerase III holoenzyme possesses:, , a) polymerase activity only, , b) 3’ 5’ endonuclease activity, , c) 3’ 5’ exonuclease activity and polymerase activities, d) 5’ 3’ exonuclease activity, , 9)In prokaryotes, the RNA primer from the lagging strand is removed and, replaced by the DNA sequence. This process is catalyzed by an enzyme ..............., which possess 5'-3' exonuclease activity and 5'-3' polymerase activity., , a) DNA polymerase |, , b) DNA polymerase I!, , c) DNA polymerase III, , d) DNA polymerase IV, , 10)The short strand of ........ primer is required for the replication of DNA:, a) DNA, , b) RNA, , c) Histone, , d) hnRNA, , 11)DNA helices exist in various form. Which of the following form is, predominantly expressed in cells, , a) A-Helix, , b) B- Helix, , c) E-Helix, , d) Z-Helix, , 12)DNA replication is bidirectional and anti-parallel. Which of the statement is, FALSE regarding the DNA replication?, , a) The DNA synthesis i.e. addition of nucleotide occurs from 5'-3' position, , b) The DNA synthesis is semi-continuous with continuous leading strand and, discontinuous lagging strand., , c) The synthesis of leading and lagging strands occurs simultaneously, , d) None of the Above