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>, , Calibration of UV / Visible Spectrophotometer, , Calibration of the UV spectrophotometer including control of absorbance using potassium dichromate solution, resolution power using toluene in hexane, limit of stray light, and wavelength accuracy., , Ensure that the connections of the spectrometer are proper., , Control of Absorbance, , Potassium Dichromate Solution, , Dry a quantity of potassium dichromate by heating to constant weight at 130°C. Weigh & transfer accurately a quantity not less than 57.0 mg & not, more than 63.0 mg to 1000 ml volumetric flask. Dissolve & dilute in sufficient 0.005M H2S04 to produce 1000 ml., , Measure the absorbance of potassium dichromate solution at the wavelengths given below., , Calculate the value of A (1% 1cm) for each wavelength., , A(1% 1cm) = Absorbance X 10000 / Weight of Potassium dichromate in mg., , Acceptance criteria, , , , , , , , , , , , Wavelengths (nm) A[1% 1cm] Limit, 235.0 124.5 122.9 - 126.2, 257.0 144.0 142.8 - 145.7, 313.0 48.6 47.0 - 50.3, 350.0 106.6 105.6 - 108.2, , , , , , , , , , , , Stringent limits are adopted from IP & BP for acceptance criteria, , Resolution power, , Record the spectrum of a 0.02% v/v solution of toluene in hexane in the range of 260 nm to 420 nm (before use check the hexane for transmittance,, using water as a blank between 260nm to 420nm & use only if transmittance is not less than 97%)., Acceptance criteria, , The ratio of the absorbance at the maximum at about 269nm to that at the minimum at about 266nm is not less than 1.5., Limit of Stray Light, , Prepare a 1.2 % wiv solution of potassium chloride in water., , Measure absorbance of the above solution at 198.0, 199.0, 200.0, 201.0, 202.0 nm using water as blank., Acceptance criteria, , Absorbance is greater than 2., , Wavelength accuracy, Resolution & Baseline flatness (inbuilt test), , Attach printer directly to the instrument (instead of the computer)., , Go to P MODE press F3 key i.e. Maintenance., , Press ‘1’., , Press Start/Stop key., , After screen changes, ensure that nothing is kept in the optical path & press the Start/Stop key again., Prints will come after all the three tests are over., , Acceptance criteria, , For wavelength accuracy: at 656.1 + 0.3 nm & at 486.0 + 0.3 nm., , Resolution: 1.0 nm or less., , Baseline flatness: + 0.002Abs., , Frequency: Once a month., , Maintenance/ Repairs, , When the instrument does not comply with the requirement/ tolerance range specified above, the instrument should be labeled “OUT OF, CALIBRATION” and should be repaired/ serviced., , After repair/ maintenance, calibrate the instrument., , REFERENCE, , Indian Pharmacopoeia, , British Pharmacopoeia, , Instruction manual of UV-1700., , CALIBRATION REPORT OF UV/ VISIBLE SPECTROPHOTOMETER, Page 1 of 2, SOP Reference — Date
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Instrument No, —, Make —, 1. Control of Absorbance:, , Potassium dichromate solution :, , , , , , , , Frequency : Once in a month, , Model , , , , , , , , , , , , , , , , , , , , , , , , , GIOSB WE. enneeecenesagenente Balance :, Tare Wt. :, Net Wt. seuaeree ml with 0.005M sulphuric acid., Dissolve and dilute 57.0 to 63.0 mg of potassium dichromate in sufficient 0.005M sulphuric acid to produce 1000.0 ml., Wavelength (nm) Absorbance (1%, 1cm) Tolerance, 235.0 122.9 to 126.2, 257.0 142.8 to 145.7, 313.0 47.0 to 50.3, 350.0 105.6 to 108.2, Calculation :, 1% Absorbance x 10000, Atom == Weight of potassium dichromate in mg, Remark : Satisfactory / Not satisfactory., Performed by : Checked by, Date : Date :, 2. Resolution power :, , 0.02% viv toluene in hexane :, , ml, , mle ml — 100.0 ml with hexane /.., Absorbance at 269 nm =, , Absorbance at 266 nm =., , , , Absorbance at 269 nm, Ratio =, Absorbance at 266 nm, (Limit : Not less than 1.5), Remark : Satisfactory / Not satisfactory., Performed by :, Date :, , — 100.0 ml with hexane, , , , Checked by:, Date, , , , CALIBRATION REPORT OF UV/ VISIBLE SPECTROPHOTOMETER, , , , , , , , , , , , , , , , , , , , , , , , Page 2 of 2, 3. Limit of stray light:, Potassium chloride solution, Gross Wt. Balance :, Tare Wt: ...., Net Wt ml with water., Dissolve and dilute accurately 1.2 g potassium chloride in sufficient water to produce 100.0 ml., Sr. No. Wavelength (nm) Absorbance Tolerance, 1 198.0, 2 199.0 ;, 3 2000 Absorbance is greater than, 4 201.0, 5 202.0, , , , , , , , Remark : Satisfactory / Not satisfactory., Performed by :, Date :, , Checked by, Date :, , , , 4. Wavelength accuracy (In built test)
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Sr. No. Wavelength Standard (nm) | Wavelength Observed Difference Limit (nm), (nm), 1 656.1 +03, 2 486.0 +03, Remark : Satisfactory / Not satisfactory., Performed by : Checked by:, Date : Date, 5. Resolution (In built test), Sr. No. Limit Observation, 1 1.0 nm or less nm, Remark : Satisfactory / Not satisfactory., Performed by : Checked by:, Date : Date, 6. Baseline flatness (In built test), Sr. No. Limit Observation, 1 + 0.002 Absorbance, Remark : Satisfactory / Not satisfactory., Performed by : Checked by:, Date : Date, Conclusion : Instrument working is Satisfactory / Not satisfactory., Next calibration due on :, Performed by : Checked by :, Date : Date :, , , , , , Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read, , pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips., Need Help: Ask Question, , Copyright © 2008-2021 Pharmaceutical Guidelines, , , , Setup | Terms of Use | FAQ
